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Nucleic Acids Research, 1977, Vol. 4, No. 3 641-648
© 1977

Articles

Endogenous DNA-directed DNA synthesising system in a microsomal fraction of embryonic chick brain

Julian Smith and Luis Soriano*

Institut d`Embryologie du CNRS et du Collége de Franca 49 bis. Avenue de la Belle-Gabrielle, 94130 Nogent-sur-Mame, France

Received January 5, 1977.

A DNA polymerising complex directed by endogenous DNA has been partially purified from 11-day-old embryonic chick brain microsomes by DEAE-cellulose and phosphocellulose column chromatography. The active fractions are eluted together with an exogenous DNA-directed DNA polymerase; after Sephadex gel filtration, the endogenous activity remains associated with a high molecular weight DNA-directed DNA polymerase. The endogenous activity of the complex has been shown to be RNase-resistant and actinomy-cin-sensitive. It requires potassium, an ATP-regenerating system and all four deoxyribonucleoside triphosphates for full activity. The significance of this activity with regard to the protovirus hypothesis is discussed.

*Present address : Departement de Biologie Moleculaire Institut Pasteur 75015 PARIS


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