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Nucleic Acids Research, 1977, Vol. 4, No. 3 685-695
© 1977

Articles

Polyamine induced aggregation of DNA

Arve Osland and Kjell Kleppe*

Department of Biochemistry, University of Bergen Bergen, Norway

*Author to whom correspondence should be addressed

Received January 12, 1977. Poly-online induced aggregation of various DNAs has been studied under conditions usually employed in many enzymatic assays where DNA is one of the substrates. Spermine was by far the most efficient polyamine in causing aggregation followed by spermidine and cadaverine. All double-stranded and naturally occurring single-stranded HCTAs were found to aggregate. No aggregation of single-stranded homodeoxypoly-mers could be detected under the same conditions. The concentration of polyamine at which the aggregation commenced was found to be a linear function of the DNA concentration. The slope of the curves depended on the nature of the polyamine, DNA the concentration of Mg++ and the ionic strength.


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