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Nucleic Acids Research, 1977, Vol. 4, No. 4 1015-1024
© 1977

Articles

Nucleotide sequence of the SV40 DNA restriction fragment Hind C - Hap 2

H. Van Heuverswyn, A. Van de Voorde and W. Fieri

Laboratory of Molecular Biology, University of Ghent Ledeganckstraat, 35 9000 Gent, Belgium

Received March 2, 1977. We here report the nucleotide sequence of the SV40 DNA fragment Hind C - Hap 2.The fragment was labeled at the 5'-ends by means of polynucleotide kinase and {gamma}-(32P)ATP and digested with a suitable restriction cntyme. The separated products were then partially degraded with the base-specific reagents dimethyl-sulphate or hydrazine followed by direct analysis on polyacryl-amide gel. The Hind C - Hap 2 sequence is 126 base pairs long and one of the three possible reading frames for translation does not contain any termination codon. So, although no protein is known to be encoded by this region, the possibility cannot yet be completely ruled out. The sequence also contains several AT-rich blocks.


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