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Nucleic Acids Research, 1977, Vol. 4, No. 4 1047-1063
© 1977

Articles

Synthesis of oligonucleotides with sequences identical with or analogous to the 3' - end of I6S ribosomal RNA of Escherichia coli: preparation of A-C-C-U-C-C via the modified phosphotriester method1

J.H. van Boom, P.M.J. Burgers, G. van der Marel, C.H.M. Verdegaal and Mrs G. Wille

Department of Organic Chemistry, Gorlaeus Laboratories, State University of Leiden P.O. Box 75, Leiden, The Netherlands

Received March 4, 1977. A combination of two different methods for the synthesis of oligoribo-nucleotides, i.e. the two-step phosphotriester method with 2-chlorophenyl phosphate as bifunctional phosphate source and the modified triester method with 2,2,2-trichloroethyl 2-chlorophenyl phosphorochloridate as monofunc-tional phosphate source, is applied for the synthesis of the fully-protected hexaribonucleotide A-C-C-U-C-C. The two-step method is used for the synthesis of the required dinucleoside monophosphates 9, 10 and 11 Application of the modified triester method for the coupling of the oligonucle-otide blocks results in the formation of the fully-protected hexamer 15. Furthermore, attention is paid to 2,4,6-triisopropylbenzsnesulphonyl 4-nitroimidazolide as a new condensing agent for the coupling of larger oligonucleotide blocks.


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