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Nucleic Acids Research, 1977, Vol. 4, No. 4 1111-1122
© 1977

Articles

Oligo(dG)12–18 aggregates result in non-homogeneity of oligo(dG)12–18-Poly(C) type primer-template

Radostawa Mikke and Barbara Zmudzka

institute of Oncology, Wawelska 15, 02-2-034 Warsaw Poland

Received March 10, 1977. Studies on the absorption spectra of equimolar solutions of oligo(dG)12–18 and poly(c), poly(Cm) or poly(ce) showed that only 13%, 3% and 3% of base pairs, respectively, farm complexes. Upon centrifugatian of oligo(dG)12–18 with a molar excess of poly(c) of poly(Cm) in an analytical and a preparative centrifuge, it was found that only a part of oligodeoxynucleotide sediments with the polynucleotide, i.e. more rapidly than oligo(dG)12–18, poly(c) or poly(Cm). Products of binding of oligo(dG)12–18 with poly(C), poly(Cm) or poly(Ce) direct the synthesis of poly(dG) by AMV reverse transcriptas in accordance with the reported characteristics of these primer.templates, as well as of the enzyme. These observations suggest that the solutions of oligo(dG)12–18 with poly(C) or its analogues, commonly used as primer .templates of RNA- and DNA-directed DNA polymerases, contain a polynucleotide, to which oligo(dG)12–18 aggregates are bound through a few nucleotides chains shorter than 12–18 residues. These chains of oligo(dG)12–18 containing the 3'-OH ends are capable of initiating the reaction with DNA polymerases.


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