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Nucleic Acids Research, 1977, Vol. 4, No. 4 813-826
© 1977

Articles

Monocistronic translation of alfalfa mosaic virus RNAs

P. Gerlinger*, E. Mohier, M.A- Le Meur* and L. Hirth

*Inst Embryol. Fac. Med. 11 rue Humann, 67000 Strasbourg, France Lab. Virol., Inst. Biol. Mol. et Cell, du C.N.R.S., Univ. Louis Pasteur 15, rue Descartes

Received December 13, 1976. The four alfalfa mosaic virus RNAs (respectively 24 S, 20 S, 17 S and 1 2 S) have been used separately as messengers in two in vitro protein synthesizing systems : wheat germ and rabbit retlculocytelysate. In both systems a polypeptlde corresponding to the translation of the entire length of the RNA can be found for RNAs 24 S, 20 S and 1 2 S, but not for 1 7 S RNA, the translation product of which is only 35,000 daltons. The number of initiation sites has been determined for each RNA by analyzing the initiation peptides synthesized in the presence of sparsomycln and show that there is only one initiation or binding site perRNA. We thus conclude that each AMV RNA behaves as a monocistronic messenger in in vitro translating systems.


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