Nucleic Acids Research, 1977, Vol. 4, No. 4 853-866
© 1977
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Salt-induced structural changes in nucleosomes
The Department of Developmental Therapeutics, The University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute Houston, TX 77030, USA
Received January 13, 1977.
Nucleosomes and oligonucleosomes were prepared by digestion of human pla-cental nuclei with staphlococccal nuclease and fractionated by gel filtration chromatography. The effect of increasing salt on the structure of nucleosomes was examined in the presence and absence of 10 mM MgCl2-. Nucleosomes and oligonucleosomes are insoluble over a broad range of salt concentration. Nucleosomes are soluble in 
120 mM (NH4)2SO4. containing 10 mM MgCl2 allowing analyses of changes in nucleosomal DNA by C.D. spectroscopy. Nucleosomes are insoluble in 
. 120 mM (NH4)2SO4. containing 10 mM MgCl2 as demonstrated by turbidity measurements. He conclude that the insolubility of nucleosomes accompanies salt-induced structural changes possibly due to individual particle condensation. As the salt concentration is increased the nucleosomes condense and then relax at higher salt concentrations.
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