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Nucleic Acids Research, 1977, Vol. 4, No. 4 899-915
© 1977

Articles

Characterization of mRNA-protein complexes from mammalian cells

Bo Sundquist+, Torgny Persson and Uno Lindberg

Department of Microbiology, The Wallenberg Laboratory, Uppsala University Uppsala, Sweden

Received January 27, 1977.

In a previous report we described the use of oligo(dT)-cellulose for the isolation of mRNA-protein complexes from EDTA-dissociated polysomes extracted from normally growing or adenovirus infected KB-cells (1). Experiments presented here provide evidence that proteins involved in these complexes bind specifically to mRNA since: a) the proteins and mRNA cosediment through sucrose gradients, b) they adsorb and elute from oligo(dT)-cellulose together, and C) analysis of the products from ribonuclease digestion experiments show that the poly(A) end and a separate small fraction of the mRNA are resistant to the enzymes and attached to protein.

+Present address: Department of Virology, The Royal Veterinary College, Biomedicum Boxn 585, S-751 23 Uppsale, Sweden


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