Nucleic Acids Research, 1977, Vol. 4, No. 4 969-987
© 1977
Articles |
Selective breakage of ONA alongside 5-bromodeoxyuridine nucleotide residues by high temperature hydrolysis
CSIRO Molecular & Cellular Biology Unit P.O.Box 90, Epping, N.S.W. 2121, Australia
Received February 17, 1977. The substitution of thymine nucleotides (pT) in ollgodeaxynucleotides by bromouracil nucleotides (pBU) changes the properties of the oligonucleotides in two ways: (1) It alters their mobility during DEAE-Cellulose homochromato-graphy1. (2) It substantially enhances their sensitivity to high temperature hydrolysis under mildly alkaline conditions (pH 8.9). The resultant breaks occur adjacent to pBU residues and leave terminal phosphates on the breakage products. With more extreme conditions some loss of terminal phosphates can occur. Heating at 100° for 16 hr at pH 8.9 produces cleavage at about half of the pBU residues with minimal loss of terminal phosphates. The properties described here may explain the thermal sensitivity of bacteria grown in 5BU2 and may have a use in DNA sequencing technology.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kamisago, S. D. Sharma, S. R. DePalma, S. Solomon, P. Sharma, B. McDonough, L. Smoot, M. P. Mullen, P. K. Woolf, E. D. Wigle, et al. Mutations in Sarcomere Protein Genes as a Cause of Dilated Cardiomyopathy N. Engl. J. Med., December 7, 2000; 343(23): 1688 - 1696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Gulick, C. B. Bauer, J. B. Thoden, E. Pate, R. G. Yount, and I. Rayment X-ray Structures of the Dictyostelium discoideum Myosin Motor Domain with Six Non-nucleotide Analogs J. Biol. Chem., January 7, 2000; 275(1): 398 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hicks, W.-M. Deng, A. D. Rogat, K. G. Miller, and M. Bownes Class VI Unconventional Myosin is Required for Spermatogenesis in Drosophila Mol. Biol. Cell, December 1, 1999; 10(12): 4341 - 4353. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Yokota, C. Yukawa, S. Muto, S. Sonobe, and T. Shimmen Biochemical and Immunocytochemical Characterization of Two Types of Myosins in Cultured Tobacco Bright Yellow-2 Cells Plant Physiology, October 1, 1999; 121(2): 525 - 534. [Abstract] [Full Text]
|
|
|
|
|
|
L. M. Coluccio and M. A. Geeves Transient Kinetic Analysis of the 130-kDa Myosin I (MYR-1 Gene Product) from Rat Liver. A MYOSIN I DESIGNED FOR MAINTENANCE OF TENSION? J. Biol. Chem., July 30, 1999; 274(31): 21575 - 21580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Raposo, M.-N. Cordonnier, D. Tenza, B. Menichi, A. Dürrbach, D. Louvard, and E. Coudrier Association of Myosin I Alpha with Endosomes and Lysosomes in Mammalian Cells Mol. Biol. Cell, May 1, 1999; 10(5): 1477 - 1494. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. J. Tyska, D. E. Dupuis, W. H. Guilford, J. B. Patlak, G. S. Waller, K. M. Trybus, D. M. Warshaw, and S. Lowey Two heads of myosin are better than one for generating force and motion PNAS, April 13, 1999; 96(8): 4402 - 4407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W Deng, K Leaper, and M Bownes A targeted gene silencing technique shows that Drosophila myosin VI is required for egg chamber and imaginal disc morphogenesis J. Cell Sci., January 11, 1999; 112(21): 3677 - 3690. [Abstract] [PDF]
|
|
|
|
|
|
M. Xiao, H. Li, G. E. Snyder, R. Cooke, R. G. Yount, and P. R. Selvin Conformational changes between the active-site and regulatory light chain of myosin as determined by luminescence resonance energy transfer: The effect of nucleotides and actin PNAS, December 22, 1998; 95(26): 15309 - 15314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Buss, J. Kendrick-Jones, C. Lionne, A. E. Knight, G. P. Cote, and J. Paul Luzio The Localization of Myosin VI at the Golgi Complex and Leading Edge of Fibroblasts and Its Phosphorylation and Recruitment into Membrane Ruffles of A431 Cells after Growth Factor Stimulation J. Cell Biol., December 14, 1998; 143(6): 1535 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Zhu, K. Beckingham, and M. Ikebe High Affinity Ca2+ Binding Sites of Calmodulin Are Critical for the Regulation of Myosin Ibeta Motor Function J. Biol. Chem., August 7, 1998; 273(32): 20481 - 20486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pinder, R. Fowler, A. Dluzewski, L. Bannister, F. Lavin, G. Mitchell, R. Wilson, and W. Gratzer Actomyosin motor in the merozoite of the malaria parasite, Plasmodium falciparum: implications for red cell invasion J. Cell Sci., January 7, 1998; 111(13): 1831 - 1839. [Abstract] [PDF]
|
|
|
|
|
|
P. Post, G. Bokoch, and M. Mooseker Human myosin-IXb is a mechanochemically active motor and a GAP for rho J. Cell Sci., January 4, 1998; 111(7): 941 - 950. [Abstract] [PDF]
|
|
|
|
|
|
K. D. Novak and M. A. Titus The Myosin I SH3 Domain and TEDS Rule Phosphorylation Site are Required for In Vivo Function Mol. Biol. Cell, January 1, 1998; 9(1): 75 - 88. [Abstract] [Full Text]
|
|
|
|
|
|
R. Mendelson and E. P. Morris The structure of the acto-myosin subfragment 1 complex: Results of searches using data from electron microscopy and x-ray crystallography PNAS, August 5, 1997; 94(16): 8533 - 8538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Martinsson, A. Oldfors, N. Darin, K. Berg, H. Tajsharghi, M. Kyllerman, and J. Wahlstrom Autosomal dominant myopathy: Missense mutation (Glu-706 right-arrow Lys) in the myosin heavy chain IIa gene PNAS, December 19, 2000; 97(26): 14614 - 14619. [Abstract] [Full Text] [PDF]
|
|