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Nucleic Acids Research, 1977, Vol. 4, No. 5 1273-1290
© 1977

Articles

A restriction endonuclease cleavage map of mouse mitochondria I DNA*

Kathleen Healy Moore{dagger}, Paul H. Johnson, Sarah E.W. Chandler and Lawrence I. Grossman

Department of Biochemistry, Wayne State University School of Medicine Detroit, Ml 48201, USA

Received February 22, 1977.

A restriction endonuclease cleavage map is presented for mouse mitochondrial DNA. This map was constructed by electron microscopic measurements on partial digests containing fixed D-loops, and by electrophoretic analysis of partial and complete single enzyme digests, and of double digests. No map differences were detected between mitochondrial DNA from cultured LA9 cells and an inbred mouse line for the six endonu-cleases used. Three cleavage sites recognized by HpaI, five sites recognized by HincII, two sites recognized by PstI and four sites recognized by BamI were located with respect to the origin of replication and the EcoRI and HinIII sites previously determined by others. No cleavages were produced by KpnI or SalI.

The migration of linear DNA with a molecular weight greater than 1 x 106 was not a linear function of log molecular weight in 1% agarose gels run at 6.6 volts/cm.

*Dedicated to Jerome Vinograd and the memory of his joy in the discovery of knowledge.

{dagger}Present address: Department of Internal Medicine, Wayne State University School of Medicine, Detroit, MI 48201.


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