Skip Navigation

This Article
Right arrow Print PDF (625K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pikoó, L.
Right arrow Articles by Matsumoto, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pikoó, L.
Right arrow Articles by Matsumoto, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 5 1301-1314
© 1977

Articles

Complex forms and replicative intermediates of mitochondrial DNA in tissues from adult and senescent mice

Lajos Pikoó and Lloyd Matsumoto

Developmental Biology Laboratory, Veterans Administration Hospital Sepulveda,CA 91343 Division of Biology, California Institute of Technology Pasadena, CA 91125, USA

Received February 4, 1977. The occurrence and types of complex forms and replicative intermediates of mitochondrial DNA (mtDNA) were investigated in tissues from C57BL/6J mice aged 10–11 months or 29–30 months. Total mtDNA from brain, heart, kidney and liver was isolated in ethidium bromide-CsCl gradients and examined by electron microscopy after aqueous or formamide spreading. Contour length measurements indicated no difference in the monomer size of mtDNA according to either tissue or donor age. The frequencies of catenated mtDNA, ranging from 4 to 8%, varied significantly according to tissue but changed relatively little as a result of donor age. The main age-related effect observed in this study was a significant increase in the frequency of circular dimers, from about 0.05% in adult tissues to 0.3% in kidney, 0.5% in liver, 0.6% in heart and 1.9% in brain of senescent mice. The frequency of D-loop DNA varied from 30 to 60% and that of larger replicative intermediates from 1 to 10%, suggesting differences in the rate of mtDNA replication according to tissue. The frequencies and types of the various replicative intermediates were unaffected by donor age.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
T. A. Brown, C. Cecconi, A. N. Tkachuk, C. Bustamante, and D. A. Clayton
Replication of mitochondrial DNA occurs by strand displacement with alternative light-strand origins, not via a strand-coupled mechanism
Genes & Dev., October 15, 2005; 19(20): 2466 - 2476.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
P. L. Kozlovskis, M. J.D. Smets, W. L. Strauss, and R. J. Myerburg
DNA Synthesis in Adult Feline Ventricular Myocytes : Comparison of Hypoxic and Normoxic States
Circ. Res., February 1, 1996; 78(2): 289 - 301.
[Abstract] [Full Text]

Home page
 HypertensionHome page
F. Inserra, L. Romano, L. Ercole, E. M.V. de Cavanagh, and L. Ferder
Cardiovascular Changes by Long-term Inhibition of the Renin-Angiotensin System in Aging
Hypertension, March 1, 1995; 25(3): 437 - 442.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.