Skip Navigation

This Article
Right arrow Print PDF (4824K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (197)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cameron, J. R.
Right arrow Articles by Davis, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cameron, J. R.
Right arrow Articles by Davis, R. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 5 1429-1448
© 1977

Articles

Analysis of chromosomal integration and deletions of yeast plasmids

John R. Cameron, Peter Philippsen and Ronald W. Davis

Department of Biochemistry, Stanford University School of Medicine Stanford, CA 94305, USA

Received January 10, 1977. Plasmid DNAs from six strains of Saccharomyces cerevisiae were compared. Three different plasmids were found, designated Scp 1, Scp 2 and Scp 3, with monomer lengths of 6. 19, 6.06 and 5.97 kilobases as referenced to sequenced ØX174 DNA. DNA from each of the plasmids was inserted into a lambda vector DNA. Hybrid phage containing inserted DNA of the desired size were enriched by genetic selection and their DNAs analysed by rapid techniques. All three plasmids share the same organization, two unique sequences separated by two inverted repeats, and share basically the same DNA sequences. Scp 2 and Scp 3 differ from Scp 1 by missing a unique Hpal site and by having small overlapping deletions in the same region. The Hpal site in Scp 1 is, therefore, in a nonessential region and suitable for insertion of foreign DNA in the potential use of the yeast plasmid as a vector. Hybridization of labelled cloned plasmid DNA to restriction fragments of linear yeast DNA separated on agarose gels showed that the plasmid DNA was not stably integrated into the yeast chromosomal DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
G. B. Morlino, L. Tizzani, R. Fleer, L. Frontali, and M. M. Bianchi
Inducible Amplification of Gene Copy Number and Heterologous Protein Production in the Yeast Kluyveromyces lactis
Appl. Envir. Microbiol., November 1, 1999; 65(11): 4808 - 4813.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
C C Kopczynski, A K Alton, K Fechtel, P J Kooh, and M A Muskavitch
Delta, a Drosophila neurogenic gene, is transcriptionally complex and encodes a protein related to blood coagulation factors and epidermal growth factor of vertebrates.
Genes & Dev., December 1, 1988; 2(12b): 1723 - 1735.
[Abstract] [PDF]

Home page
 ScienceHome page
V. Parikh, M. Morgan, R Scott, L. Clements, and R. Butow
The mitochondrial genotype can influence nuclear gene expression in yeast
Science, January 30, 1987; 235(4788): 576 - 580.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.