Skip Navigation

This Article
Right arrow Print PDF (1803K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (109)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mickel, S.
Right arrow Articles by Bauer, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mickel, S.
Right arrow Articles by Bauer, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 5 1465-1482
© 1977

Articles

Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs{dagger}

Susan Mickel, Vincent Arena, Jr. and William Bauer

Department of Microbiology, Health Sciences Center, State University of New York Stony Brook, NY 11794, USA

Received February 23, 1977.

A series of closed circular (I) plasmid DNAs has been derived from drug resistance factor R12, and the nicked circular (II) and linear (III) derivatives of these molecules prepared by irradiation in the presence of ethidium bromide and by treatment with restriction enzyme EcoRI, respectively. These DNAs encompass the molecular weight range 3.6 to 61 megadaltons. The base compositions range from 45% to 51% (GC) as estimated by buoyant density determinations. The smaller plasraids are significantly less super-coiled (9–10%) than are the larger (12–13%). The gel electrophoretic behavior of the three DNA structural forms was determined as a function of molecular weight in agarose gels of concentrations ranging from 0.7% to 1.6% and at electrophoresis salt concentrations from 0.02 M to 0.08 M sodium acetate. The mobilities of DNAs I and III undergo a reversal relative to each other at a molecular weight which decreases with increasing agarose gel concentration. The molecular weight at which DNA II fails to enter a gel depends upon the ionic strength during electrophoresis but not upon the gel concentration.

{dagger}This paper is dedicated to the memory of Professor Jerome Vinograd, our dear friend and teacher.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. M. Robertson and D. E. Smith
Strong effects of molecular topology on diffusion of entangled DNA molecules
PNAS, March 20, 2007; 104(12): 4824 - 4827.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Viglasky, M. Antalik, J. Adamcik, and D. Podhradsky
Early melting of supercoiled DNA topoisomers observed by TGGE
Nucleic Acids Res., June 1, 2000; 28(11): e51 - e51.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
E. Garvey and D. Santi
Stable amplified DNA in drug-resistant Leishmania exists as extrachromosomal circles
Science, August 1, 1986; 233(4763): 535 - 540.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.