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Nucleic Acids Research, 1977, Vol. 4, No. 5 1505-1512
© 1977

Articles

Isolation of folded chromosomes from Mycoplasma hyorhinis*

Marlyn Teplitz

Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena, CA 91125, USA

Received February 1, 1977.

Folded chromosomes were isolated from Mycoplasma hyorhinis. When examined by electron microscopy, these molecules show variability of loop size, number of loops, total contour length and degree of twisting of the DNA. Sedimentation velocity was unaltered after treatment with RNase, proteinase K, SDS, temperatures up to 65°C and NaCl concentrations from 0.1 M to 4 M.

*Most of these experiments were done under the supervision of Dr. Jerome Vinograd. The manuscript was writtem after his death and is dedicated to his memory.


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