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Nucleic Acids Research, 1977, Vol. 4, No. 5 1609-1632
© 1977

Articles

Complementary addressed modification of yeast tRNA1Val with alkylating derivative of d(pC-G)-A. The positions of the alkylated nucleotides and the course of the alkylation in the complex*

N.I. Grineva, G.G. Karpova, L.M. Kuznetsova, T.V. Venkstern and A.A. Bayev

Inst. Org. Chem., Siberian Branch of the Academy of Sciences of the USSR Novosibirsk Inst. Mol. Biol., Academy of Sciences of the USSR Moscow, USSR

Received January 6, 1977.

Yeast Formula alkylation with 2'.3'– 0–4–(N-2-chloroet-hyl-N-methylamino) benzylidene d(pC-G;-A proceeds at 20°-30°C in the complementary complexes which are formed by d(pC-G)-A>RC1 binding to 3 sequences of Formula:{Psi}-C-G58 in the D loop, C-G40 at the 3'-side of the anticodon loop and C-G-18 in the D loop. The reaction in the complexes results in A53, I35 and {Psi}13 alkylation to form B-/N-methyl-N-(formylphenyl)amino-ethyl Formula with the relative rate constants of the alky-lation that are 3 or 2 orders of magnitude higher than that for the alkylation without a complex formation. It is the third nucleotide from the 5'-terminus of the binding site of the modifying agent that is subjected to alkylation in the Formula. The course of the alkylation does not depend on the possible base pairing of the 3'-terminal nucleotide of the reagent. The extent of the reagent binding and the relative rate constants of the alkalytion in the complexes indicate the following order of the complex stability: ({Psi}-C-G58)>C-G40){approx}(C-G18) at 20° and ({Psi}-C-G58)>(C-G40)>(C-G18) at 30°

*Dedicated to the memory of Jerome Vinograd.


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