Skip Navigation

This Article
Right arrow Print PDF (519K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (204)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pearson, W.R.
Right arrow Articles by Britten, R.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pearson, W.R.
Right arrow Articles by Britten, R.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 6 1727-1738
© 1977

Articles

A program for least squares analysis of reassociation and hybridization data

W.R. Pearson, E.H. Davidson and R.J. Britten

Division of Biology, California Institute of Technology Pasadena, CA 91125, USA

Received December 21, 1976. A computer program is described for the rapid calculation of least squares solutions for data fitted to different functions normally used in reassociation and hybridization kinetic measurements. The equations for the fraction not reacted as a function of Cot follow: First order, exp(–kCot); second order, (1+kCot)–1 ; variable order, (1+kCot)–n; approximate fraction of DNA sequence remaining single stranded, (1+kCot)–44; and a function describing the pairing of tracer when the rate constant for the tracer (k) is distinct from the driver rate constant kd): exp{kL1-(1+kdCot)1–nJ/Lkd(1-n)J}. Several components may be used for most of these functional forms. The standard deviations of the individual parameters at the solutions are calculated.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
J. Gans, M. Wolinsky, and J. Dunbar
Computational Improvements Reveal Great Bacterial Diversity and High Metal Toxicity in Soil
Science, August 26, 2005; 309(5739): 1387 - 1390.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
D. E. Neafsey and S. R. Palumbi
Genome Size Evolution in Pufferfish: A Comparative Analysis of Diodontid and Tetraodontid Pufferfish Genomes
Genome Res., May 1, 2003; 13(5): 821 - 830.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. Carr and G. Shearer Jr.
Genome Size, Complexity, and Ploidy of the Pathogenic Fungus Histoplasma capsulatum
J. Bacteriol., December 15, 1998; 180(24): 6697 - 6703.
[Abstract] [Full Text]

Home page
 ScienceHome page
W. Timberlake
Low repetitive DNA content in Aspergillus nidulans
Science, December 1, 1978; 202(4371): 973 - 975.
[Abstract] [PDF]

Home page
 ScienceHome page
R. Wallace, S. Dube, and J Bonner
Localization of the globin gene in the template active fraction of chromatin of Friend leukemia cells
Science, December 16, 1977; 198(4322): 1166 - 1168.
[Abstract] [PDF]

Home page
 Genome Res.Home page
D. G. Peterson, S. R. Schulze, E. B. Sciara, S. A. Lee, J. E. Bowers, A. Nagel, N. Jiang, D. C. Tibbitts, S. R. Wessler, and A. H. Paterson
Integration of Cot Analysis, DNA Cloning, and High-Throughput Sequencing Facilitates Genome Characterization and Gene Discovery
Genome Res., May 1, 2002; 12(5): 795 - 807.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.