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Nucleic Acids Research, 1977, Vol. 4, No. 6 1759-1768
© 1977

Articles

Nucleotide sequence neighbouring a late modified guanylic residue within the 28S ribosomal RNA of several eukaryotic cells

Marthe-Elisabeth Eladari, Annie Hampe and Francis Galibert

Laboratoire d'Heématologie Expeérimental, Centre Hayem Hoôpital Saint-Louis, 2 Place du Dr Fournier, 75475 - Paris Cedex 10, France

Received January 25, 1977. The nucleotide sequence of a particular T1 oligonucleotide found in 4 IS and 28S RNAs of several cellular cell lines (human, mouse, rat and chicken fibroblast) but absent in 45S ribosomal RNA has been deduced. Its primary structure : A-U-U-G-{psi}-U-C-A-C-C-C-A-C-U-A-A-U-A-Gp shows the presence of a modified G residue which explains the existence of this oligonucleotide in the T1 fingerprint of 41S RNA and 28S RNA. Its absence on the 45S RNA T1. fingerprint is accounted for by a late modification.


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