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Nucleic Acids Research, 1977, Vol. 4, No. 6 1793-1802
© 1977

Articles

In vitro transcription of herpes simplex virus ANG DNA by E. coli RNA polymerase

Giinter P. Strauss, Inge B. Maichle, Rita Schatten and Hans Christian Kaerner++

Institute of Virus Research, German Cancer Research Center Heidelberg, GFR

Received January 28, 1977.

HSV-1 ANG DNA and a defective genome of the same virus were transcribed with E. coli RNA polymerase under various salt conditions. The extent of transcription was assayed by hybridizing the cRNA to the Hind III, Hpa I and Hind II restriction fragments of the DNA templates using the blot technique of E. Southern. The transcripts proved to contain sequences homologous to all DNA fragments. A similar ratio of hybridized cRNA and the amount of fragment DNA was observed in all cases. The results suggest that both, the wt and the defective HSV ANG genome were completely transcribed.

++Address inquiries to Dr. Hans C. Kaerner, Deutsches Krebs forscnungszentrum, Institut für Virusforschung, Postfach 1019119,0 6900 Heidelberg, PRO.


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