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Nucleic Acids Research, 1977, Vol. 4, No. 6 1933-1944
© 1977

Articles

Poly-2'-deoxy-2'-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acid

W. Guschlbauera, M. Blandina, J.L Drocourtb and M.N. Thangb

aSery.Biochim., Bat.142. Centre D'Etudes Nuclfiaires de Saclay B.P.No.2, F-91190-Gif-sur-Yvette bInst.Biol.Physico-chim. 13, Rue Pierre et Marie Curie, F-75005 Paris, France

Received March 15, 1977. The polymerization of 2'deoxy-2'-fluoro-cytidine-diphosphate (dCflDP) by polynucleotide phosphorylase is barely detectable in the presence of Mg++ under usual experimental conditions for polymerization of nucle-oside diphosphates. High concentrations of enzyme have to be used to accomplish the synthesis. Mn++ is a better activator than Mg++ for the reaction. dCflDP inhibits the polymerization of CDP and has Km=8.8x10–3M,six times higher than CDP. - The polymer, poly(dCfl), ressembles in many respects poly(C), but not poly(dC): the acid selfstructure forms at similar pK's; in-teraction with poly(I) yields a 1:1 complex the CD spectrum of which is similar to that of poly(I).poly(C). Finally, the Formulas of polyd).poly(dCfl) are comparable to those of poly(I).poly(C).


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