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Nucleic Acids Research, 1977, Vol. 4, No. 6 2039-2056
© 1977

Articles

Chromatin core particle unfolding induced by tryptic cleavage of histones

D.M.J. Lilley*,1 and K. Tatchell2

1Searle Res. Lab. Lane End Road, High Wycombe, Bucks, HP12 4HL, UK 2Dep. Biochem. and Biophys., Oregon State Univ. Corvallis, OR 97331, USA

Received April 4, 1977.

Chromatin ‘core particles’ have been digested with trypsin to varying extents. The resulting particles are homogeneous by the criterion of ultracentrifuge boundary analysis. Sedimentation coefficients are lowered as cleavages are introduced into the histones, showing that an unfolding of the core particle occurs. This unfolding is further characterised by a lower melting temperature together with a premelting phase, higher molar ellipticity in the circular dichroism spectra at 280 nm and increased kinetics of digestion by both micrococcal nuclease and DNase I. Differences are also observed in the products of nuclease digestion. The most consistent interpretation of the data involves an unfolding process whereby free rods of DNA are released to extend from a nucleoprotein core.

* To whom reprint requests should be addressed.


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