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Nucleic Acids Research, 1977, Vol. 4, No. 7 2137-2144
© 1977

Articles

DNA sequence at the end of the cI gene in bacteriophage {lambda}

Zafri Humayun

New York University School of Medicine, Department of Biochemistry 550 First Avenue, New York, NY 10016, USA

Received February 28, 1977. The nucleotide sequence of 57 base pairs near the end of the cI gene in phage {lambda} is presented. This sequence was determined by direct sequencing techniques and includes the codons for 11 carboxyterminal aminoacids of the cI product, the {lambda} repressor. The sequence reveals that the cI gene, which has recently been shown to have a unique initiation region, is terminated by a UGA codon. A GUG triplet, which could act as a translational start signal for the rex gene occurs 8 base pairs beyond the cI termination codon. This GUG triplet is preceded by a sequence that could serve as a strong ribosome binding site for the rex message.


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