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Nucleic Acids Research, 1977, Vol. 4, No. 7 2283-2292
© 1977

Articles

A rapid cytosine modification of E. coli tRNA1Leu by semicarbazide-bisulfite, a probe for polynucleotide conformations

Kazuo Negishi1, Fumio Harada2, Susumu Nishimura2 and Hikoya Hayatsu1

1Faculty of Pharmaceutical Sciences, University of Tokyo Bunkyo-ku, Tokyo 2National Cancer Center Research Institute Tsukiji, Chuo-ku, Tokyo, Japan

Received April 26, 1977. Cytosine residues in 32P-labeled E. coli tRNALeu were modified by treatment of the tRNA with the semicarbazide-bisulfite reagents [Hayatsu, H. (1976) Biochemistry 15, 2677–2682]. Analysis of the modification sites showed that only four cytidine residues, i.e. C35 C53 C85 and C86 reacted. They were identical with the cytidines of this tRNA accessible to methoxyamine [Chang, S. E. and Ish-Horowicz, D. (1974) J. Mol. Biol. 84, 375–388] and the accessibility was consistent with the conformational features recognized for tRNA in general.

The rapidity and the simple nature of this modification demonstrate that the semicarbazide-bisulfite reaction is a useful tool in studying conformations of polynucleotides.


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