Skip Navigation

This Article
Right arrow Print PDF (1970K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wilson, J. T.
Right arrow Articles by Weissman, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilson, J. T.
Right arrow Articles by Weissman, S. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 7 2353-2368
© 1977

Articles

Nucleotide sequence of 3' untranslated portion of human alpha globin mRNA

J. T. Wilson, J. K. deRiel, B. G. Forget, C. A. Marotta* and S. M. Weissman

Department of Human Genetics and Internal Medicine, Yale University School of Medicine New Haven CT 06510 *Department of Psychiatry, Harvard Medical School and Mailman Research Center McLean Hospital. Belmont. MA 02178. USA

Received May 3, 1977. We have determined the nucleotide sequence of 75 nucleotides of the 3'-untranslated portion of normal human {alpha} globin mRNA which corresponds to the elongated amino acid sequence of the chain termination mutant Hb Constant Spring. This was accomplished by sequence analysis of cDNA fragments obtained by restriction endonuclease or T4 endonuclease IV cleavage of human globin cDNA synthesized from globin mRNA by use of viral reverse transcriptase. Analysis of cRNA synthesized from cDNA by use of RNA polymerase provided additional confirmatory sequence information. Possible polymorphism has been identified at one site of the sequence. Our sequence overlaps with, and extends the sequence of 43 nucleotides determined by Proudfoot and coworkers for the very 3'-terminal portion of human {alpha} globin mRNA. The complete 3'-untranslated sequence of human a globin mRNA (112 nucleotides including termination codon) shows little homology to that of the human or rabbit ß globin mRNAs except for the presence of the hexanucleotide sequence AAUAAA which is found in most eukaryotic mRNAs near the 3' poly(A).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.