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Nucleic Acids Research, 1977, Vol. 4, No. 7 2369-2388
© 1977

Articles

The repeating nucleotide sequence in the repetitive mitochondrial DNA from a "low-density" petite mutant of yeast

C. F. Van Kreijl and J. L. Bos

Section for Medical Enzymology and Molecular Biology, Laboratory of Biochemistry, University of Amsterdam, Eerste Constantijn Huygensstraat 20, Amsterdam, Netherlands

Received May 4, 1977. The repeating nucleotide sequence of 68 base pairs in the mtDNA from an ethjdjum-jnduced cytoplasmic petite mutant of yeast has been determined. For sequence analysis specifically primed and terminated RNA copies, obtained by in vitro transcription of the separated strands, were used. The sequence consists of 66 consecutive AT base pairs flanked by two GC pairs and comprises nearly all of the mutant mitochondrial genome. The sequence, more over, also represents the first part of wild-type mtDNA sequenced so far.


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