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Nucleic Acids Research, 1977, Vol. 4, No. 7 2421-2428
© 1977

Articles

A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs

R. Giegé*, B. Jacrot**, D. Moras***, J. C. Thierry*** and G. Zaccai**,{dagger}

*Lab. Chim. Biol., Inst. Biol. Mol. et Cell. du CNRS 15, rue Descartes 67084 Strasbourg Cédex **Inst. Laue-Langevin B.P. No.156X, 38042 Grenoble Cédex ***Lab. Cristallochim., Univ. Louis Pasteur B.P. 296 R/8,67008 Strasbourg Cédex, France

{dagger}To whom all correspondence should be addressed

Received May 9, 1977. A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with its cognate and non cognate yeast tRNAs. It was shown that, when limited amounts of tRNA (either cognate or non cognate) are added to valyl-tRNA synthetase, a complex consisting of two enzyme molecules and one tRNA molecule is first formed. It is subsequently dissociated to a one to one complex when more tRNA is present in the solution. The association curve shows a maximum for a molecular ratio, enzyme over tRNA, equal to 2.


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