Nucleic Acids Research, 1977, Vol. 4, No. 8 2747-2756
© 1977
Articles |
NMR spectroscopy of the ring nitrogen protons of uracil and substituted uracils; relevance to A
base pairing in the solution structure of transfer RNA
Biochemistry Department, University of California Riverside, CA 92502, USA
Received May 9, 1977.
The chemical shifts of the well-resolved ring nitrogen protons of uracil and a series of substituted uracils, including pseudouridine (5-ribosyl-uracil) and uridine (1-ribosyl-uracil) were determined using nuclear magnetic resonance (NMR). The observed chemical shifts suggest the existence of an atypical syn conformation for pseudouridine in the A
base pair in regulatory tRNAs in solution.