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Nucleic Acids Research, 1977, Vol. 4, No. 8 2767-2777
© 1977

Articles

Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s

J. T. Sági, A. Szabolcs, A. Szemzö and L. Ötvös

Central Research Institute for Chemistry of the Hungarian Academy of Sciences H-1525 Budapest, P. O. Box 17, Hungary

Received May 16, 1977. The chemical synthesis of 5-alkyl-dUTP-s and their participation as substrates in poly[d(A-T)] primed polymerization reactions with dATP by E. coli DNA polymerase I enzyme has been described. In comparison with dTTP, at saturating substrate concentrations, the rate of hypochromic effect was found to be 17.3 % higher for dUTP and was lower by 27.4 % for 5-ethyl-dUTP, 29.5 % for 5-n-propyl-dUTP, 31.4 % for 5-n-butyl-dUTP and by 85.0 % for 5-n-pentyl-dUTP. No hypochromic effect could be observed, however, with 5-iso-propyl-, 5-tert.butyl- and 5-n-hexyl-dUTP-s. Polydeoxynucleotides have also been isolated from the reaction mixture and some of their structural properties determined.


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