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Nucleic Acids Research, 1977, Vol. 4, No. 8 2871-2879
© 1977

Articles

Relation between Escherichia coli endonucleases specific for apurinic sites in DNA and exonuclease III*

Siv Ljungquist and Tomas Lindahl

Department of Chemistry, Karolinska Institute 104 01 Stockholm, Sweden

Received June 8, 1977.

Contradictory data have recently been published from two different laboratories on the presence vs absence of an intrinsic endonucleolytic activity of E. coli exonuclease III at apurinic sites in double-stranded DNA. It is shown here that an endonuclease activity of this specificity co-chromatographs exactly with exonuclease III on phosphocellulose and Sephadex G-75 columns, indicating that the endonuclease and exonuclease activities are due to the same enzyme. In addition, another E. coli endonuclease specific for apurinic sites exists, which can be separated from exonuclease III by the same chromatographic procedures.

* This investigation was supported by the Swedish Natural Science Research Council, the Swedish Cancer Society, and the Karolinska Institute.


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