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Nucleic Acids Research, 1977, Vol. 4, No. 9 3143-3154
© 1977

Articles

Preferential binding of S-phase proteins to temporally-characteristic units of replication in Physarum polycephalum

John J. Wille, Jr.

Department of Zoology and Physiology, Louisiana State University Baton Rouge, LA 70803. USA

Received June 24, 1977. Synchronous plasmodia of Physarum polycephalum were pulse-labeled with 3H-thymidine in early or late portions of the S-phase, and the binding capacity of the replicated DNA for isochronous S-phase plasmodial proteins assessed by nitrocellulose filter binding assay. Replication units replicating during the first one-third of the S-phase preferentially bind cytosol proteins present in plasmodia engaged in early S DNA replication, while late S replicating DNA exhibits a corresponding preferential binding of plasmodial proteins present only in late S plasmodia. Temporally-characteristic nascent replication units were isolated by Hydroxylapatite column chromatography and were found to contain binding sites for isochronous proteins.


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