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Nucleic Acids Research, 1978, Vol. 5, No. 1 105-115
© 1978

Articles

The location of the globin mRNA sequence within its 16S precursor

Kate Smith, Paul Rosteck, Jr. and Jerry B. Lingrel

Department of Biological Chemistry, University of Cincinnati College of Medicine Cincinnati, OH 45267, USA

Received September 23, 1977. The coding sequence of globin mRNA has been located at or very close to the 3' end of its poly(A)-containing 16S precursor. The 16S RNA was annealed to globin cDNA and the hybrid digested with ribonuclease H. The undigested fragment did not bind to oligo(dT)-cellulose and its size was that expected for the intact 5' portion of the precursor.


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