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Nucleic Acids Research, 1978, Vol. 5, No. 1 13-21
© 1978

Articles

Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography

Hubert Eshaghpour and Donald M. Crothers

Yale University, Department of Chemistry New Haven, CT 06520, USA

Received September 29, 1977. High pressure reversed phase chromatography (RPC-5) at pH 12 was used for preparative separation of the complementary strands of the smaller DNA fragments which are generated by the Hae III restriction endonuclease digestion of Col E1 DNA. A single high pressure RPC-5 chromatographic step at neutral pH served to purify duplex fragments 70, 172, 250 and 440 base pairs long; each of these yielded two elution peaks upon chromatography under alkaline denaturing conditions.


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