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Nucleic Acids Research, 1978, Vol. 5, No. 1 161-171
© 1978

Articles

The interaction of ethidium with synthetic double-stranded polynucleotides at low ionic strength

Bruce C. Baguley* and Ellen-Marie Falkenhaug

Department of Cell Biology, University of Auckland Private Bag, Auckland, New Zealand

*Address correspondence to B.C. Baguley, c/o CIBA GEIGY Ltd., Room K-125.7.42, CH-4002, Basel, Switzerland.

Received October 18, 1977. The interaction of ethidiuin with synthetic DNA and RNA double-stranded polymers at 0.01 M ionic strength, pH 7.0, has been studied by fluorimetry at low drug to nucleotide ratios. Binding constants have been calculated assuming an excluded-neighbouring site model for the interaction of ethidium with double-stranded polymers. The values obtained are poly d(AT).poly d(AT), 9.5x106 M–1 ; poly dA poly dT, 6.5x106 M–1 poly d(GC).poly d(GT), 9.9x106 M–1; poly dG.poly dC, 4.5x106 M–1; poly d(AC).poly d(GT), 9.8x106 M–1; poly d(AG).polyd(CT), 1.3x106 M–1; poly rA.poly rU, 4.1x107 M–1. The displacement of ethidium from poly d(AT) .poly d(AT) by 9-aininoacridine and an acridine-containing antitumour agent (NSC 156303; 4'-(9-acridinylamino)methanesulphon-m-anisidide) has also been examined.


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