Skip Navigation

This Article
Right arrow Print PDF (2813K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Vennström, B.
Right arrow Articles by Philipson, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vennström, B.
Right arrow Articles by Philipson, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 1 205-219
© 1978

Articles

Initiation of transcription in nuclei isolated from adenovirus infected cells

Björn Vennström, Ulf Pettersson and Lennart Philipson

Department of Microbiology, Biomedical Center, Uppsala University Uppsala, Sweden

Received October 31, 1977. Initiation of adenovirus transcription was analyzed by incubation of isolated nuclei from virus infected cells in the presence of ß–32P GTP or ß–32P ATP. Nucleotide analysis of RNA from nuclei incubated with ß–32P GTP shows that the label is incorporated exclusively into pppGp and ppGp. Under similar incubation conditions, the label from ß–32P ATP was incorporated primarily into the 5' phosphate of 5', 3' mononucleoside diphosphates, but label was also detected in pppAp, pppGp and in the 3'' nucleoside monophosphates. Analysis of RNA, synthesized in the presence of different concentrations of {alpha}–amanitin, shows that only RNA polymerase III initiates virus specific transcription in isolated nuclei. The virus specific transcripts containing pppAp and pppGp in their 5' termini were identified as the 5.5S and 5.2S viral RNA species by hybridization and finger printing.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.