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Nucleic Acids Research, 1978, Vol. 5, No. 1 271-284
© 1978

Articles

Turbidimetric and potentiometric studies of ribosomal subunits from an erythromycin resistant mutant of Escherichia coil

J. S. Kliber, G. Hui Bon Hoa*, M. Grunberg-Manago**, D. Pardo+ and R. Rosset+

Institut de Biologie Physico-chimique Groupe U128, INSERM, 75005 Paris, France *Institution de Biologie Physico-chimique 13, rue Pierre et Marie curie, 75005 Paris, France **Institut de Biologie Physico-chimique, Service de Biochimie 75005 Paris, France +centre de Biochimie et de Biologie Moléculaire CNRS, 13009 Marseille, France

Received November 22, 1977. Turbidimetric and potentiometric techniques were applied to the analysis of an EryR mutant. Results show that in the mutant, the 3OS subunits are drastically altered, as indicated by a higher Mg2+ requirement for subunit association and by an important difference in the titratable groups. Replacement of parental 5OS by mutant 5OS subunits does not decrease the association capacity with 3OS parental subunits, but a structural difference is detected in the mutant 5OS with potentiometric measurements. The mutation results in decreased ribosomal in vitro activities at 22°C including lowered polyphenyl-alanine synthesis, drastic altered initiation step and the loss of erythromycin binding to the ribosomes.

The results extend previous observation of a gene eryC part in the maturation of both subunits.


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