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Nucleic Acids Research, 1978, Vol. 5, No. 1 87-103
© 1978

Articles

Oestradiol-receptor complexes in subnuclear fractions of rat uterine tissue

Willem de Boer, Joan de Vries, Eppo Mulder and Henk J. van der Molen

Department of Biochemistry (Division of Chemical Endocrinology), Medical Faculty, Erasmus University Rotterdam Rotterdam, Netherlands

Received September 6, 1977. The subnuclear distribution of 3H-oestraiol-receptor complexes was studied in uterine tissue of ovariectomized adult rats. Nuclei were sonically disrupted and 8 different subnuclear fractions were isolated by discontinuous sucrose density gradient centrifugation. 3H-oestraiol-receptor complexes, measured by hydroxylapatite column chromatography, were localized in a light chromatin fraction as well as in a heavy chromatin fraction. Using the hydroxylapatite chromatography technique it was possible to demonstrate three classes of oestradiol-receptor complexes which differ in affinity for the chromatin. Oestradiol-receptor complexes with a high affinity for the chromatin were predominantly localized in the heavy chromatin fraction, whereas complexes with a lower affinity for their acceptor sites were present in the lighter chromatin fraction.


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