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Nucleic Acids Research, 1978, Vol. 5, No. 10 3469-3478
© 1978

Articles

SV40 viral minichromosome: preferential exposure of the origin of replication as probed by restriction endonucleases

A.J. Varshavsky*, O.H. Sundin and M.J. Bohn

Department of Biology, Massachusetts Institute of Technology Cambridge, MA 02139, USA

*Address for correspondence: A.J.Varshavsky, Room 56-642, Department of Biology, Massachusetts Institute of Technology, 77 Mass. Ave., Cambridge, MA 02139, USA

Received August 10, 1978. Isolated SV40 minichromosomes [1–3] were treated with different single-cut restriction endonucleases to probe the arrangement of nucleosomes in relation to the SV40 DNA sequence. While Eco RI and Bam HI each cut 22–27% of the SV40 minichromosomes under limit-digest conditions, Bgl I, which cuts SV40 DNA at or very near the origin of replication [4,5], cleaves 90–95% of the minichromosomes in a preparation. Similar results were obtained with minichromosomes which had been fixed with formaldehyde before endonuclease treatment. One possible interpretation of these findings is that the arrangement of nucleosomes in the compact SV40 minichromosomes is nonrandom at least with regard to sequences near the origin of DNA replication.


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