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Nucleic Acids Research, 1978, Vol. 5, No. 10 3491-3502
© 1978

Articles

Patterns of codon recognition by isoacceptor aminoacyl-tRNAs from wheat germ

Dolph Hatfield and Mary Rice

Nucleic Acids Section, Chemistry Branch, National Cancer Institute, National Institutes of Health Bethesda, MD 20014, USA

Received August 3, 1978. Isoacceptors of Ala-, Arg-, Glu-, Gln-, Ile-, Leu-, Lys-, Ser-, Thr-and Val-tRNAs from wheat germ have been resolved by reverse phase chromatography. Codon recognition properties have been determined on isolated fractions of each of these aa-tRNAs and codon assignments have been made to a number of isoacceptors. Evolutionary changes which have occurred in patterns of codon recognition by isoacceptor aa-tRNAs in wheat germ and other organisms are discussed.


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