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Nucleic Acids Research, 1978, Vol. 5, No. 10 3515-3522
© 1978

Articles

The nucleotide sequence of the 5' untranslated region of human {gamma}-globin mRNA

Judy C. Chang*,{dagger}, Raymond Poon*,+, Kurt H. Neumann* and Yuet Wai Kan*,+

*Hematology Service, San Francisco General Hospital, University of California San Francisco +Howard Hughes Medical Institute Laboratory, San Francisco General Hospital San Francisco, CA, USA

{dagger}To whom correspondence should be addressed at San Francisco General Hospital, San Francisco, California, 94110.

Received August 1, 1978. The nucleotide sequence of the entire 5' untranslated region of human {gamma}-globin mRNA has been determined. This was accomplished by analyzing complementary DNA (cDNA) synthesized form the mRNA with reverse transcriptase. The cDNA was labeled at its 3' end with 32P using terminal deoxynucleotidyl transferase, digested with the restriction endonuclease Hae III and the end-labeled fragment isolated and sequenced by the method of Maxam and Gilbert. Including the initiation codon AUG, the 5' untranslated region of human {gamma}-globin mRNA contains 57 nucleotides, compared to 4l in {alpha}- and 54 in ß- globin mRNA. There is very little homology between {alpha} and {gamma} sequences in the 5' region. There is considerable homology between ß- and {gamma}-globin mRNAs in the regions proximal and distal to the initiation codon, but the entire sequence shows less homology than the human and rabbit ß-globin mRNAs. The hexanucleotide sequence CUUCUG is found near the 5' ends of all three human globin mRNAs, suggesting a possible role of this sequence in ribosomal binding. Both guanosine and cytidine were found at the 19th nucleotide position from the 5' end of the {gamma} mRNA. We believe this heterogeneity arises from the difference in nucleotide sequence between the A{gamma} and G{gamma} loci.


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