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Nucleic Acids Research, 1978, Vol. 5, No. 10 3913-3928
© 1978

Articles

Pulsed FT-NMR double resonance studies of yeast tRNAPhe: specific nuclear Overhauser effects and reinterpretation of low temperature relaxation data

Paul D. Johnson* and Alfred G. Redfield{dagger}

*Department of Biochemistry, Brandeis University Waltham, MA 02154, USA {dagger}Departments of Biochemistry and Physics and Rosenstiel Basic Medical Sciences Research Center, Brandeis University Waltham, MA 02154, USA

Received July 10, 1978. Cross-relaxation effects are demonstrated between the imino protons and other protons in yeast tRNAPhe and H2O. A detailed examination has been made of the observed relaxation rate of the proton resonance at 11.8 ppm from DSS as a function of the D2O content in the solvent. This result, as well as the size and number of observed nuclear Overhauser effects, suggests that dipolar magnetization transfer between solvent H2O, amino, imino, and other tRNA protons may dominate the relaxation processes of the imino protons at low temperature. At higher temperatures the observed relaxation rate is dominated by chemical exchange.

The selective nuclear Overhauser effects are shown to be an important aid in resonance assignments. By these means we were able to identify two protons from the wobble base pair GU4 at 11.8 ppm and 10.4 ppm.


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