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Nucleic Acids Research, 1978, Vol. 5, No. 11 4025-4038
© 1978

Articles

Formation and characterization of antibody against 2'-(5''-phosphoribosyl)-5' AMP, the monomer form of poly (adenosine diphosphate ribose)

Harutake Sakura, Masanao Miwa, Yoshiyuki Kanai*, Taijiro Matsushima* and Takashi Sugimura

Virology Division, National Cancer Center Research Institute Tsukiji 5-1-1, Chuo-ku, Tokyo 104 *Department of Molecular Oncology, Institute of Medical Science, University of Tokyo Minato-ku, Tokyo 108, Japan

Received August 24, 1978. Specific antibody against 2'-(5''-phosphoribosyl)-5'ANP (PR-AMP), a monomer of poly (adenosine diphosphate ribose) (poly(ADP Rib)), was produced by immunizing a rabbit with PR-AMP coupled to bovine serum albumin (BSA). Antibody against PR-AMP was purified 53-fold from serum by (NH4) 2SO4 precipitation, and BSA-Sepharose 4B, DEAE-cellulose and (PR-AMP)-BSA-Sepharose 4B column chromatographies. Inhibition experiments show that the adenine ring, 5'-phosphate residue and ribose-ribose bond of PR-AMP were essential for the antigenic determinant of PR-AMP. Anti PR-AMP antibody bound, not only with PR-AMP, but also with poly(ADP-Rib) of various chain lengths, while anti poly(ADP-Rib) antibody bound with poly(ADP-Rib) but not with PR-AMP.


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