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Nucleic Acids Research, 1978, Vol. 5, No. 11 4155-4164
© 1978

Articles

Partial purification of transcriptionally active nucleosomes from trout testis cells

Beatriz Levy W. and Gordon H. Dixon

Division of Medical Biochemistry, The University of Calgary Calgary, Alberta, T2N 1N4, Canada

Received August 1, 1978. Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These inonosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.


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