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Nucleic Acids Research, 1978, Vol. 5, No. 11 4329-4342
© 1978

Articles

Isopentenyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae

Howard Laten, John Gorman and Robert M. Bock

Laboratory of Molecular Biology and Department of Biochemistry, University of Wisconsin Madison, WI 53706, USA

Received August 16, 1978. We have isolated a mutant of Saccharomyces cerevisiae that contains 1.5% of the normal tRNA-complement of isopentenyladenosine (i6A). The mutant was characterized by the reduction in efficiency of a tyrosine inserting UAA nonsense suppressor. The chromatographic profiles of tRNATer tRNASer and on benzoylated DEAE-cellulose are consistent with the loss of i6A by these species. Transfer RNA from the mutant exhibits 6.5% of the cytokinin biological activity expected for yeast tRNA. Transfer RNAs from the mutant that normally contain i6A accept the same levels of amino acids in vitro as the fully modified species. With the exception of i6A, the level of modified bases in unfractionated tRNA from the mutant appears to be normal. The loss of i6A apparently affects tRNA's role in protein synthesis at a step subsequent to aminoacylation.


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