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Nucleic Acids Research, 1978, Vol. 5, No. 12 4631-4642
© 1978

Articles

DNA methylation at a CCGG sequence in the large intron of the rabbit ß-globin gene: tissue-specific variations

C. Waalwijk and R.A. Flavell

Section for Medical Enzymology and Molecular Biology, Laboratory of Biochemistry, University of Amsterdam P.O.Box 60.000, 1005 GA Amsterdam, The Netherlands

Received September 19, 1978. We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit ß-globin gene. In most somatic tissues, including erythroid and non-erythroid tissues, about 50% of the DNA is resistant to cleavage at this site by HapII, though 100% cleavage is found with the isoschizomer MspI. Since the former enzyme is unable to cleave CCGG sites if the internal C residue is 5-methyl C (and since methylation is the only form of CpG modification documented in animal DNA), while the latter enzyme cleaves DNA irrespective of methylation at this residue, we infer that 50% of the CCGG sites in the ß-globin gene intron are methylated in these tissues.

The same site appears to be 100% methylated (judged by the same criterium) in sperm DNA and about 80% methylated in brain DNA. DNA from the rabbit SIRC cell line is entirely unmethylated at this site.


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