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Nucleic Acids Research, 1978, Vol. 5, No. 12 4725-4736
© 1978

Articles

Detection of yeast ribosomal RNA sequences in E. coli infected with hybrid bacteriophage

Richard A. Kramer and Joan Smallwood

Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health Bethesda MD 20014, USA

Received August 24, 1978. Yeast ribosomal DNA was inserted into Escherichia coli on a bacteriophage vector and the host cell RNA was then extracted and analyzed for the presence of yeast ribosomal RNA sequences. RNA complementary to yeast rDNA was detected by hybridization. The transcription of yeast rDNA was found to be independent of phage RNA synthesis and to occur on the same DNA strand as rRNA transcription in yeast. However, hybridization to restriction fragments of yeast rDNA suggested that the RNA species detected in E. coli differ somewhat from authentic yeast rRNA.


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