Skip Navigation

This Article
Right arrow Print PDF (519K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Jost, J.-P.
Right arrow Articles by Cozens, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jost, J.-P.
Right arrow Articles by Cozens, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 12 4781-4794
© 1978

Articles

Identification of a large preculsor of vitellogenin mRNA in the liver of estradiol-treated chicks

Jean-Pierre Jost, Gundula Pehling, Takeshi Ohno and Peter Cozens

Friedrich Miescher-Institut P.O. Box 273, CH-4002 Basel, Switzerland

Received October 19, 1978. Studies were performed to determine whether vitellogenin mRNA from avian liver has a precursor molecule or not. Total cellular RNA was prepared from estradiol-treated chicken liver in the presence of 8 M guanidine HCl, 2-mercaptoethanol and aurintricarboxylic acid. After denaturation, RNA was fractionated on sodium dodecylsulfate-sucrose gradients and large size RNA was analyzed under stringent conditions on 85% formamide-sucrose gradients at 25°C. RNA fractions collected from the gradients were hybridized with vitellogenin (3H)-cDNA Besides mature vitellogenin mRNA (32S, 7,000 nucleotides) vitellogenin sequences were also found in RNA fractions ranging from 38–50S with a peak at 45–50S (12–15,000 nucleotides). Only 5–10% of the putative 38–50S pmRNA is polyadenylated. We calculated that the half-life of vitellogenin pmRNA is about 3–4 minutes. We conclude that vitellogenin mRNA has a precursor which is twice the size of the mature mRNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.