Skip Navigation

This Article
Right arrow Print PDF (372K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (38)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pollock, J. M.
Right arrow Articles by J.Herbert, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pollock, J. M., Jr.
Right arrow Articles by J.Herbert, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 12 4855-4864
© 1978

Articles

Methylation of DNA in early development: 5-methyl cytosine content of DNA in sea urchin sperm and embryos

James M. Pollock, Jr., Marcia Swihart and Taylor J.Herbert

Institute of Molecular Biophysics and Department of Biological Science, Florida State University Tallahassee, FL 32306, USA

Received September 18, 1978. By separating formic acid hydrolysates with high pressure chromatography on an Aminex-10 column, we determined the ratio of 5-methyl cytosine to cytosine and other bases of DNA from sea urchin sperm and nuclei of embryos from early cleavage through pluteus stages. Contrary to several previous reports, we could not find any measurable changes in the methylation levels of embryonic nuclear DNAs at different stages of development. We also found no consistent differences between the methylation levels of sea urchin sperm and embryonic nuclei or the 5-methyl cytosine content of fish (Mugil cephalus) sperm and liver nuclei. While these measurements would not have detected subtle variations associated with differentiation, they would have indicated the gross changes previously reported for embryos or between sperm and somatic nuclei had those changes been present.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
M Ehrlich and R. Wang
5-Methylcytosine in eukaryotic DNA
Science, June 19, 1981; 212(4501): 1350 - 1357.
[Abstract] [PDF]

Home page
 ScienceHome page
A Razin and A. Riggs
DNA methylation and gene function
Science, November 7, 1980; 210(4470): 604 - 610.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.