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Nucleic Acids Research, 1978, Vol. 5, No. 2 435-450
© 1978

Articles

Overmethylation of DNAs by the EcoRI methylase

Kathleen L. Berkner and William R. Folk

Department of Biological Chemistry, The University of Michigan Ann Arbor, MI 48109, USA

Received December 22, 1977. EcoRI methylase is able to catalyze methyl incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI* endonuclease activity, and thus is presumably at or near the EcoRI* sequences (5') NAATTN.


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