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Nucleic Acids Research, 1978, Vol. 5, No. 2 537-548
© 1978

Articles

Nucleotide sequence of threonine tRNA from Bacillus subtilis

Tsunemi Hasegawa and Hisayuki Ishikura

Laboratory of chemistry, Jichi Medical School, Minamikawachi-machi Tochigi-ken, 329-04, Japan

Received November 23, 1977. A threonine tRNA was purified from Bacillus subtilis W168 by a combined use of column chromatographic systems. The nucleotide sequence was determined to be pG-C-C-G-G-U-G-U-A-G-C-U-C-A-A-U-D-G-G-D(U)-A-G-A-G-C-A-A-C-U-G-A-C-U-mo5U-G-U-t6A-A-{Psi}-C-A-G-U-A-G-m7G-U-U-G-G-G-G-G-T-{Psi}-C-A-A-G-U-C-C-U-C-U-U-G-C-C-G-G-C-A-C-C-AOH, where about 40% of D20 remained unmodified as U20. It consists of 76 nucleotides including a new minor nucleoside, 5-methoxyuridine (mo5U) which occupies the wobble position of anticodon.


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