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Nucleic Acids Research, 1978, Vol. 5, No. 3 637-651
© 1978

Articles

Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells

Karel Grohmann, François Amalric, Stephen Crews and Giuseppe Attardi

Division of Biology, California Institute of Technology Pasadena, CA 91125, USA

Received November 15, 1977. The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml camptothecin, and selected for poly(A) content by two passages through oligo(dT)-cellulose, was digested either with the nuclease P1 or with a mixture of RNases: the digestion products were then fractionated by two- dimensional electrophoresis. No "cap" structures were detected under conditions where the presence of such structures in one out of five to ten RNA molecules would have been recognized. It is, therefore, likely that "cap" structures are completely absent in HeLa cell mitochondrial poly(A)-containing RNA.


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