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Nucleic Acids Research, 1978, Vol. 5, No. 3 697-712
© 1978

Articles

Complexity of poly(A+) and poly(A–) polysomal RNA in mouse liver and cultured mouse fibroblasts

Leo J. Grady, Arlene B. North and Wayne P. Campbell

Division of Laboratories and Research, New York State Department of Health Empire State Plaza, Albany, NY 12201, USA

Received December 1, 1977. RNA excess hybridization experiments were used to measure the complexity of nuclear RNA, poly(A+) mRNA, poly(A–) mRNA, and EDTA-released polysomal RNA sedimenting at <8O S in mouse liver and in cultured mouse cells. With both cell types, poly(A–) RNA was found to contain 30–40% of the sequence diversity of total mRNA. In the case of liver this represents 5,700 poly(A–) molecules and 8,600 poly(A+) molecules for a total of approximately 14,300 different mRNAs. Comparison of the complexity of mRNA with that of nuclear RNA revealed that in liver and in cultured cells, mRNA has only 10–20% of the sequence diversity present in nuclear RNA. This latter observation is consistent with existing data on mammalian cells from this and other laboratories.


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